The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. 
For general laboratory use.

Product No：07800029001     
Pack Size：200 x 20 µL reactions  
Product No：07800096001     
Pack Size：1000 x 20 µL reactions   包

Keywords: Hydrolysis Probes  
 
 
Overview概述：
The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
Ease of use, convenience, speed, reduced error
Result consistency, stability and tolerance of targets of varying secondary structure
Ability to detect low copy or rare transcripts for gene expression applications
Superior quantification capability for gene expression applications
Easier assay optimization
Contamination control capability
Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
Obtain fast and specific results


Contents内容
Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, and proprietary additive
反应混合物，5× conc.包含逆转录和PCR酶，RT-qPCR反应缓冲酶，dATP, dCTP, dGTP, dUTP, Mg(OAc)2和专有添加剂

07 800 029 001   2 vials, 440 μL each
07 800 029 001    2瓶，每瓶 440 μL

07 800 096 001 10 vials, 440 μL each
07 800 096 001    10瓶，每瓶440μL

Water, PCR grade To adjust the final reaction volume.
水，PCR 级 调整最终反应体积。

07 800 029 001   3 vials, 1 mL each
07 800 029 001    3瓶，每瓶 1 mL

07 800 096 001   2 vials, 7.25 mL each
07 800 096 001    2瓶，每瓶 7.25 mL

Application应用：

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.
In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).
The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.
There are protocols for both the LightCycler® 480 instrument and the LightCycler® 96 instrument.
1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.