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罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master

罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master

品牌:
罗氏/Roche
市场价:¥0.00

The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler ® EvoScript RNA SYBR ® Green I Master专 为敏感、高 特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。

For general laboratory use.供一般实验室使用。
Product No:07800134001   产品编号:07800134001  
Pack Size:200 x 20 µL reactions  包装尺寸:200 x 20 µL 反应
Product No:07800177001  产品编号:07800177001   
Pack Size:1000 x 20 µL reactions   包装尺寸:1000 x 20 µL 反应
Keywords: SYBR Green  关键词:SYBR 绿色
 
 
概述:
The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
LightCycler®EvoScript RNA Probes Master(1 瓶组合物)专为灵敏、高特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。
 
Ease of use, convenience, speed, reduced error
Result consistency, stability and tolerance of targets of varying secondary structure
Ability to detect low copy or rare transcripts for gene expression applications
Superior quantification capability for gene expression applications
Easier assay optimization
Contamination control capability
Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
Obtain fast and specific results

  1.  
  2. 适用简单、方便 快捷、减少错误
  3. 结果表明,不同二级结构的目标具有一致性、稳定性和耐受性
  4. 能够检测低拷贝或 转录本的基因表达应用
  5.  基因表达应用定量能力
  6. 更容易分析优化
  7. 污染控制能力
  8. 生成一致且可  靠的结果,无论您从什么样本类型开始或检测什么指标
  9. 在检测低拷贝数时依靠更高的灵敏度,将更大的样品体积输入到反应中
  10. 获得快 速和具体的结果
Contents
Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, SYBR® Green I dye and proprietary additives
07 800 134 001   2 vials, 440 μL each
07 800 177 001  10 vials, 440 μL each
Water, PCR grade To adjust the final reaction volume.
07 800 134 001   3 vials, 1 mL each
07 800 177 001   2 vials, 7.25 mL each
内容
  1. 包含用于逆转录和 PCR 的酶、RT-qPCR 反应缓冲液、dATP、dCTP、dGTP 和 dUTP、Mg(OAc) 2、SYBR ®  Green I 染料和专有添加剂
  2. 07 800 134 001    2瓶,每瓶 440 μL
    07 800 177 001    10瓶,每瓶440μL
  1. 水,PCR 级 调整最终反应体积。
    07 800 134 001    3瓶,每瓶 1 mL
    07 800 177 001   2瓶,每瓶 7.25 mL
 
 
应用:
The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.
LightCycler ®  EvoScript RNA SYBR ®  Green I Master 专为灵敏、高特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。1瓶组合物非常适合简单的反应组装,只需要添加引物和目标 RNA。专 有的反应缓冲液允许方便的热启动 RT-qPCR。
 
In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).
The master mix is optimized for SYBR® Green I applications and does not require optimization with Mg(OAc)2.
1)The reverse transcription activity will be suppressed at temperatures significantly below 60°C.
 
此外,用于逆转录和 PCR 的酶具有热启动特性,具有 特异性1)。
预混液针对 SYBR ®  Green I 应用进行了优化,不需要使用 Mg(OAc) 2进行优化。
1)在显着低于 60°C 的温度下,逆转录活性将被抑制。
 

属性 参数
产品名称 规格型号 简介/参数 市场价
罗氏试剂 Roche EvoScript Universal cDNA Master 07912374001 Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA SYBR 07912498001 罗氏试剂 Roche EvoScript RNA SYBR Green I Master (ROX) The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays on instruments requiring ROX dye. For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA Probes Master 07912269001 The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. EvoScript RNA Probes Master 专为灵敏、高 特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。 For general laboratory use. 供一般实验室使用。 0.00
罗氏/roche LightCycler® DNA Master HybProbe 12015102001 Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments. 易于使用的 qPCR 试剂盒,适用于所有基于LightCycler ® 转盘的仪器的HybProbe 信号传输。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® Fluorescein CPG 03138178001 3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments. 在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis KitLightCycler® Red 610-N-hydroxysuccinimide ester 03561488001 Blue solid, for a minimum of 250 nmol oligonucleotides 蓝色固体,至少 250 nmol 寡核苷酸 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。  0.00
罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase 03539806001 LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA. 0.00
罗氏试剂 Roche LightCycler ® Multiplex DNA Master 07339577001 Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems. 易于使用的5倍反应混合优化的多重qPCR,与LightCycler®480或LightCycler®96实时PCR系统兼容。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® FastStart DNA Master HybProbe 03003248001 Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System. 易于使用的热启动反应混合物,用于使用 LightCycler ® 基于旋转木马的系统进行 PCR 。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis Kit 06366821001 Reagent for lysing cells prior to two-step real-time RT-PCR applications. For life science research only. Not for use in diagnostic procedures. 0.00
罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. LightCycler ® EvoScript RNA SYBR ® Green I Master专 为敏感、高 特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。 0.00

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